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. Author manuscript; available in PMC: 2012 Feb 8.
Published in final edited form as: Biochemistry. 2011 Jan 13;50(5):820–827. doi: 10.1021/bi101483r

Fig. 4. Effect of glycerol stimulation on crosslinking efficiency.

Fig. 4

UU1581 cells carrying pPA818 (Tar-6his) or pCS12 (Tsr) derivatives with single-cysteine substitutions were grown to mid-log phase under conditions of physiological induction of receptors (10 µM IPTG for Tar and 0.45 µM sodium salicylate for Tsr), washed twice and resuspended in phosphate buffer. TMEA treatment was performed 10 seconds after adding water (−) or 1 M glycerol (G) to the cell suspension.