Table 2. Crosslinking between cysteine reporters located at close or distant levels in different receptors.
UU1581 cells expressing cysteine-substituted Tsr and Tar receptors at physiological levels were treated with TMEA and the crosslinking products were analyzed by immunoblotting with an anti-Tsr antibody that reacts with both receptors.
| Position in Tar | Position in Tsr | Distances a | Tar-Tsr mixed crosslinking products b | |
|---|---|---|---|---|
| 2-subunits | 3-subunits | |||
| 436 (II) | 440 (III) | 20.97, 22.73 | + | + |
| 430 (II) | 440 (III) | 19.41, 26.72 | + | − |
| 366 (I) | 433 (II) | 30.61, 31.50 | − | − |
| 430 (II) | 366 (I) | 27.08, 29.82 | − | − |
| 436 (II) | 447 (V) | 27.62, 30.75 | − | − |
a
The table summarizes the distances between the alpha carbon from the corresponding residue in Tar to each of the alpha carbon residues in two differently substituted Tsr dimers in a mixed trimer.