Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2011 Jan 7;10(2):269–278. doi: 10.1158/1535-7163.MCT-10-0728

Figure 6.

Figure 6

Impact of attenuation of p53R2 on DNA damage in KB cells. A, neither attenuation nor overexpression of p53R2 modulated p53 protein level (p53 protein was developed by immunoprecipitation-Western blot; mouse and rabbit anti-p53R2 antibodies were used for pull-down and blot respectively). B, overexpression of p53R2 significantly increased the luciferase activity of p21 promoter reporter: p21-Luc (* P < 0.05), but not the luciferase activity of p53 reporter plasmid PG-13. C, q-RT-PCR and D, Western-blot analysis showed that the attenuation of p53R2 did not change p53 accumulation induced by adramycin, but significantly decreased p21 expression in KB cells. Under both normal and stressed conditions, attenuation of p53R2 substantially enhanced the DNA damage in KB cells, which was indicated by increased phosphorylation of γ-H2AX and phosphorylated ATM proteins.