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. 2011 Mar;25(3):990–1001. doi: 10.1096/fj.10-163261

Figure 2.

Figure 2.

Loss of SERCA2 impairs temporal sequence of desmoplakin border translocation. A) Time-lapse single-plane images of A431 cells expressing DP-GFP and transiently transfected for 72 h with siRNA oligos targeting either GAPDH (top panels) or SERCA2 (bottom panels) coming into contact following scratch wounding. Arrows indicate a newly forming cell-cell border. Inset: boxed region highlights cytoplasmic DP-GFP particles in a “beads-on-a-string” pattern that fail to incorporate into the assembling junction. B) Retrospective immunostaining of the imaged cells for SERCA2 to confirm SERCA2 expression in the siGAPDH-treated cells (top panel) and SERCA2-deficiency in the siSERCA2-treated cells (bottom panel). C) Quantitation of DP intensity at assembling cell-cell borders in cells treated with siGAPDH oligos (solid line with diamonds; n=12 movies) or siSERCA2 oligos (dashed line with squares; n=23 movies). *P < 0.05; Student's t test. Error bars = se. Scale bars = 10 μm.