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. 2011 Mar;25(3):990–1001. doi: 10.1096/fj.10-163261

Figure 3.

Figure 3.

Desmoplakin aligns along keratin filaments and is retained in the cytoplasm on loss of SERCA2 or PKCα. SCC9 cells transiently transfected for 72 h with siRNA targeting either GAPDH (A), SERCA2 (B), or PKCα (C) were subjected to a 3-h calcium switch, double-immunostained using antibodies against DP (green) and keratin-18 (red), and imaged at ×100 by confocal microscopy. All images are of a single confocal plane. Images in right panels are split-channel ×3 zoomed versions of areas outlined in white dots in left panels. Linescan analyses (graphs in right panels) are derived from the zoomed images and depict the pixel intensity of DP (blue trace) and keratin-18 (red trace) within that region. Arrows at bottom indicate sites of cell-cell contact. Asterisks above each point in graphs represent sites of one-to-one correlation of DP to keratin-18. Images and linescans shown are representative of >50 cell borders across ≥3 experiments. Scale bars = 20 μm.