Figure 7.

PKCα stimulation rescues the aberrant DP-IF alignment observed following SERCA2 knockdown. NHEK (A) or SCC9 (B) cells were transiently transfected for 72 h with siRNA targeting either GAPDH or SERCA2 and double-immunostained for DP (green) and keratin 18 (red) following a 3-h calcium switch. NHEKs were treated with DMSO or 1 μM PMA for 30 min prior to the calcium switch. SCC9s were stably expressing an empty vector or CA PKCα. Images in bottom panels are 3× zoomed versions of areas outlined in white dots in top panels. All images were collected at ×100 and are representative of >20 analyzed single-plane confocal borders for each condition. Scale bars = 10 μm.