Figure 1.

Generation of transgenic hTERT reporter mice. A) Schematic illustration of the BAC reporter. BAC clone RPCI11–117B23 was used, in which an Rluc cassette was inserted at the hTERT ATG codon. Exons are designated as vertical lines. Arrows indicate transcriptional directions of the CRR9, hTERT, and Xtrp2 genes. B) Analysis of transgenic reporter locus. Top panel: diagram of the BAC reporter. Bottom panel: characterization of transgenic founders. Genomic DNAs were digested with SpeI and subjected to pulsed-field gel electrophoresis, followed by Southern blotting hybridized to probe E (47) and Rluc ORF. C) Identification of transgenic founder lines. Tail genomic DNAs were digested with PvuII and analyzed by Southern blot analysis using a mixture of Rluc and mTERT promoter probes. Numbers at bottom are copy numbers calculated based on the relative intensities of Rluc and mTERT fragments. BAC, BAC construct; M, molecular mass marker.