Figure 2.

Expression of transgenic hTERT reporters. A) Luciferase expression in adult tissues. Rluc activities in 2-mo-old A-line and D-line mice were normalized to the amounts of total proteins. B) mRNA expression in adult tissues. Total RNAs were extracted from tissues of multiple 2- to 4-mo-old D-line mice and normal human tissues. mRNA expressions were determined by qRT-PCR in triplicates, normalized to 18S rRNA. mTERT and Rluc mRNA levels in left and middle panels are expressed as percentage of those in iPSC clone III-9. Right panel shows relative levels of hTERT mRNA. *ND, not determined.