Figure 3.

In vivo expression of the transgenic hTERT reporter. A) Whole-mount in situ RNA hybridization of Rluc and mTERT mRNAs in developing embryos. E9.5 and E10.5 transgenic (D-line) and wild-type embryos were hybridized to antisense and sense riboprobes of Rluc and mTERT, as indicated. B) Whole-body BLI detection of Rluc expression in a D-line adult animal. C) Reporter expression in testis by RNA in situ hybridization. All panels except c are consecutive cryosections of a testis from a D-line male; panel c is from a nontransgenic wild-type mouse. a–e) RNA in situ hybridization using antisense (a, c, d) and sense (b, e) riboprobes of Rluc (a–c) and mTERT (d, e). g, h) Magnified images of brackets in a (g) and f (h). Dotted lines outline one seminiferous tubule. Arrowheads indicate basal layer of spermatogonial stem cells; arrows demarcate elongating spermatids. Weak Rluc signals around feet, rectal area, and oral nasal area likely resulted from autofluorescence of dried urine. Scale bars = 200 μm.