Figure 2.

Subcellular localization of endogenous Fyn with nuclear export inhibitor and tyrosine kinase inhibitor. A) HepG2 cells were pretreated with 100 μM genistein for 2 h; cells were then treated with either DMSO or 100 μM t-BHQ along with genistein for indicated time points. Lysate was immunoblotted with anti-Fyn. B) HepG2 cells were pretreated with 20 ng/ml of LMB for 2 h; cells were then treated with either 100 μM DMSO or t-BHQ along with LMB for indicated time points. Lysate was immunoblotted with anti-Fyn. Comparison between DMSO and t-BHQ treated time points shows a significant difference. **P < 0.005; 2-tailed Student's t test.