Figure 2.

SUnSET measurements of enhanced protein synthesis in the absence of changes in rRNA. Serum-starved C2C12 myoblasts were stimulated with or without 100 nM insulin (INS) for 90 min, and rates of protein synthesis were measured with the SUnSET technique described in Materials and Methods. A) Representative image of WB analysis for puromycin followed by Coomassie Blue staining to verify equal loading of proteins. B) Quantification of the puromycin-labeled peptides (n=8 wells/group from 3 independent experiments). RLU, relative light units. C) Relative concentration of 28S and 18S rRNA per cell culture well (n=6 wells/group from 3 independent experiments). All values are expressed as a percentage of the values obtained in control samples and are presented as means + se. *P < 0.05 vs. control.