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. 2011 Mar;25(3):1028–1039. doi: 10.1096/fj.10-168799

Figure 5.

Figure 5.

In vivo overexpression of CA-PKB induces an increase in protein synthesis as determined with IV-IHC-SUnSET. TA muscles were transfected with plasmid DNA encoding the GFP (A–C) or HA-tagged CA-PKB (D–F). At 3 d after transfection, muscles were subjected to IV-IHC-SUnSET by staining for puromycin and GFP or the HA tag, respectively. A) Representative image of the signal for GFP positive fibers. B) Grayscale image of the signal for puromycin in the same section shown in A. D) Representative image of the signal for CA-PKB positive fibers. E) Grayscale image of the signal for puromycin in the same section shown in D. C, F) Puromycin staining intensity in both transfected (positive) and nontransfected (negative) fibers, expressed relative to the mean value obtained in nontransfected (negative) fibers from the same section; values are plotted on histograms for GFP-transfected muscles (C) and CA-PKB-transfected muscles (F). Values next to group labels in legend are means ± se; n = 180 transfected and nontransfected fibers/group from 3 independent muscles. *P < 0.05 vs. control.