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Retarded growth and branching of ureteric buds in Fz4−/−;Fz8−/− mouse kidneys. (A-F) At E11.5, immunolocalization of Pax2 and E-cadherin (A,B) and in situ localization of Ret and Wnt11 transcripts (C-F) reveal retarded growth of ureteric buds in the Fz4−/−;Fz8−/− kidney. (G-J) At E12.5, there is impoverished branching of ureteric buds in the Fz4−/−;Fz8−/− kidney. Fz4−/−;Fz8−/− and Fz4+/+;Fz8−/− littermate samples were processed in parallel. Scale bars: 100 μm.
