Fig. 6.

Transcriptome changes in response to loss of Fz4 with or without Fz8. (A,B) Mouse MOE430 2.0 Affymetrix arrays were hybridized in three biologically independent experiments with RNA from E13.5 Fz4^−/−;Fz8^−/−, Fz4^+/+;Fz8^−/−, Fz4^−/− and WT kidneys (two to six kidneys per RNA sample). Probe sets representing Ret, Gfra1 and Gdnf transcripts show reduced hybridization with RNA from Fz4^−/−;Fz8^−/− kidneys. As expected, the probe set representing Fz4 transcripts shows reduced hybridization with RNA from both sets of mutant kidneys. Insets in A show in situ hybridization of E11.5 kidneys, showing reduced levels of Gdnf transcripts in Fz4^−/−;Fz8^−/− relative to Fz4^+/+;Fz8^−/− kidneys.