Fig. 2.

Zic1 and Zic4 have dynamic expression patterns throughout cerebellar development. (A,B) P1 cerebellar sagittal sections: Zic1 and Zic4 were expressed in the cerebellar external granule layer (egl, arrow) and white matter (wm), and Zic4 was also expressed in the internal granule cell layer (igl, arrowhead). (C,D) Anterior view of whole cerebellum at E17.5; Zic1 and Zic4 were broadly expressed in the embryonic cerebellum. d, dorsal; v, ventral; a, anterior; p, posterior; m, medial; l, lateral. (E-H) Whole-mount lateral (E,G) and dorsal (F,H) views of the E12.5 cerebellar region (cb, brackets) with dotted lines (F,H) indicating the level of E12.5 sagittal sections in I,J. (I,J) Zic1 and Zic4 were both expressed in the cerebellar rhombic lip (rl) and mesenchyme (mes), with Zic1 also expressed in the ventricular zone (vz). Scale bars: 500 μm in A-H; 100 μm in I,J.