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. Author manuscript; available in PMC: 2011 Feb 22.
Published in final edited form as: Nat Med. 2010 Mar 28;16(4):406–412. doi: 10.1038/nm.2110

Fig. 3.

Fig. 3

IFN-β treatment blocks TH1 induced EAE but exacerbates TH17 induced EAE. (a and b) Clinical scores from mice with passive EAE induced by adoptive transfer of (a) TH1 and (b) TH17 cells that were treated with rmIFN-β or PBS every second day from day 0 to 10 post transfer (n=9 to 11 mice per group). *P<0.05. c) Histology of spinal cord sections from TH1 and TH17 induced EAE treated with IFN-β or PBS. Sections of spinal cord were obtained 45 days after transfer and stained with H&E and Luxol fast blue. Scale bars, 50 μm. (d–f) Frequency of CD4+ lymphocytes expressing IFN-γ (d), IL-17 (e) and IL-10 (f) in the spinal cords 45 day post transfer. The mean percentage ± standard deviation (N=3 experiments) of cytokine positive cells is given. Each experiment is a pool from 2–3 mice per group. (g–i) Concentration of IFN-γ (g), IL-17 (h) and IL-10 (i) from supernatants of MOGp35–55 stimulated spleens taken from mice 45 days post transfer. Data represent mean and standard deviation of 3–4 mice per group. *P<0.05.