Table 3.
Linear regression analysis of cell area, perimeter and single segment changes from 3 DAG to 5 DAG using time-lapse images
| Field | R2 (area) (1) | R2 (perimeter) | R2 (segments) | IF (%) (2) | Growth rate (%/hour) (3) |
|---|---|---|---|---|---|
| 1 (N = 6) | 0.999 | 0.999 | 0.963 ± 0.030 (4) | 91 ± 3 (5) | 1.89 ± 0.26 (6) |
| 2 (N = 4) | 0.998 | 0.996 | 0.991 ± 0.007 | 89 ± 4 | 1.11 ± 0.18 |
| 3 (N = 5) | 0.975 | 0.992 | N.D. (7) | 88 ± 2 | 1.42 ± 0.24 |
(1) R2 represents the R-squared values in linear regression analysis of surface area, cell perimeter, and anticlinal wall segment lengths plotted for populations of cells at 3 and 5 DAG. All p values are smaller than 0.01 during regression analysis.
(2) IF: Isotropy Factor is calculated as the overlap between digitally isotropically amplified 3 DAG cells and the real cell imaged at 5 DAG.
(3) Growth rate was calculated as ((5 DAG area - 3 DAG area)/(3 DAG area * 48))*100%
(4), (5) , (6) Mean ± SD
(7) N.D. Not determined