Table 3.
Effect of BMP4 and Noggin added to in vitro maturation medium on development of parthenogenic and in vitro fertilized embryos
| Treatment | Total | Cleaved (%) | Blastocysts (%) | Hatching blast. (%) | Blastocyst cell number ± sd | |
|---|---|---|---|---|---|---|
| Control | 344 | 190 (55.2)b | 30 (8.7) | 100 ± 33 | ||
| PA | BMP4 | 274 | 180 (65.7)a | 22 (8.0) | 1(0.4) | 88 ± 14 |
| Noggin | 242 | 158 (65.3)a | 22 (9.0) | 68 ± 8 | ||
| Control | 249 | 176 (70.7)α | 35 (14.0) | 5 (2.0) | 90 ± 25 | |
| IVF | BMP4 | 221 | 160 (72.4)α | 28 (12.6) | 4 (1.8) | 120 ± 25 |
| Noggin | 234 | 144 (61.5)β | 31(13.2) | 2 (0.8) | 99 ± 8 |
Development of parthenogenic (PA) and in vitro fertilized (IVF) embryos produced from oocytes that were matured in the presence of 100 ng/ml of BMP4 or Noggin. Controls were matured in standard medium without supplementation. Different superscripts in PA or IVF indicate statistical differences (Chi-square test, p < 0.05). For blastocyst cell numbers, one-way ANOVA was applied