Table 5.
Effect of BMP4 and Noggin added to culture medium on development of parthenogenic and in vitro fertilized embryos
| Treatment | Total | Cleaved (%) | Blastocysts (%) | Hatching blast. (%) | Blastocyst cell number ± sd | |
|---|---|---|---|---|---|---|
| Control | 354 | 237 (66.9)a | 48 (13.5)a | 68 ± 33 | ||
| PA | BMP4 | 295 | 199 (67.4)a | 44 (14.9)a | 1 (0.3) | 91 ± 43 |
| Noggin | 269 | 154 (57.2)b | 19 (7.0)b | 71 ± 16 | ||
| Control | 218 | 138 (63.3)α | 45(20.6)α | 10 (4.6)α | 130 ± 47 | |
| IVF | BMP4 | 217 | 146 (61.3)αβ | 22 (9.2)β | 3 (1.4)β | 117 ± 52 |
| Noggin | 205 | 105 (51.2)β | 24 (11.7)β | 1(0.5)β | 128 ± 21 |
Development of parthenogenic (PA) and in vitro fertilized (IVF) embryos cultured with 100 ng/ml of either BMP4 or Noggin, Controls were cultured without supplementation. Different superscripts in PA or IVF indicate statistical differences Chi-square test (p < 0.05). For blastocyst cell numbers, one-way ANOVA was applied.