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SDS PAGE of supernatants from different time points during fermentation. Aliquots of supernatants from different points of time (S-0 h to S-73 h) were analyzed by SDS-PAGE and subsequent Coomassie staining (A) and Western blot (B). In the Coomassie stained gel a prominent band at about 60 kDa became clearly visible which is also detectable by the L-19 antibody (B). No signals were detected before induction (S-0 h) and with a supernatant from a WT culture (S-WT).
