Figure 5. Biochemical characterization of cell surface ligands.
A) C. albicans cells treated with proteinase K or NaIO4 followed by interaction with H34 clone showed binding insensitive to the proteinase treatment. M13 used as a negative control showed no interaction with untreated or treated cells. Cells were visualized at 800X magnification. B) Presence of excess of some monosaccharides inhibits the peptide- cell interactions. Phage clones incubated with excess (1 M) of Glucose (D), Galactose (G) Mannose (M), and N acetyl glucosamine (N) were allowed to interact with C. albicans yeast cells and the extent of interaction (or fold inhibition) was determined by plaque assay. C) Inhibition by excess monosaccharides is concentration and isomer dependent. In the same assay as in B, Mannose or α−D–methyl mannopyranoside used at 1 M and galactose or mannose at 10−4 M concentration and inhibition was determined as above. Values shown represent an average of three readings.