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. 2011 Feb 22;6(2):e17160. doi: 10.1371/journal.pone.0017160

Figure 2. IL-1β/IL-23-polarized cells express Th17 markers and produce IL-17A.

Figure 2

(A) Naive CD4+ T cells were cultured for 6 d in the presence of anti-CD3/CD28 coated beads, with or without IL-1β. On d6 cells were counted and IL-2 added to each condition, together with IL-1β and IL-23, where required. IL-1β/IL-23-polarized Th17 cells were collected after 13 d of culture for phenotypic analysis or rested and assayed for Th17 cytokine on d14/15. (B) mRNA expression levels of RORC, IL-23R and CCR6 on d13 were significantly increased in IL-1β/Il-23-polarized cells. (C) IL-17A production on d15 was significantly enhanced in IL-1β/Il-23-polarized cells. Results are representative of 5 experiments. Each symbol corresponds to one individual, horizontal bars represent medians. Wilcoxon signed rank test was performed. ** P<0.01, ***P<0.001.