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. 2011 Feb 22;6(2):e17362. doi: 10.1371/journal.pone.0017362

Figure 3. Redistribution of c-Myb during the cell cycle.

Figure 3

Jurkat cells were fixed with formaldehyde, stained with Hoechst 33342, fractionated into cell cycle fractions by fluorescence-activated cell sorting, then chromatin from equal numbers of cells in different cell cycle fractions was analyzed by ChIP using anti-Myb antibodies. The association of c-Myb with known target genes (A) CCNB1, (B) CCNE1 or (C) CXCR4 is shown. Results are expressed as fold enrichment relative to control IgG, and normalized to a control site in the GAPDH promoter, to which c-Myb does not bind. Error bars show standard deviation of triplicate QPCR reactions. These results are representative of several independent experiments. Note: The experiments in this figure were performed more than five times and two different anti-Myb antibodies were used for the ChIP assays, which gave similar results. The results shown are from a single experiment but are representative of all the trials.