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. 2011 Feb 22;6(2):e17362. doi: 10.1371/journal.pone.0017362

Figure 4. Expression of cyclin target genes correlates with c-Myb binding.

Figure 4

Asynchronously growing Jurkat cells were collected, stained and sorted into G1 or combined S/G2/M phase fractions, then RNA was isolated and analyzed for the expression of the (A) CCNB1 or CXCR4 or (B) CCNE1 gene mRNAs using quantitative real-time PCR. The results were normalized to housekeeping genes GAPDH and PPIA and to the expression levels detected in the asynchronously growing cells, which were arbitrarily set to 1.0. Note: The experiments in this figure were performed at least twice. The results shown are from a single experiment but are representative of all the trials.