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. 2011 Feb 22;6(2):e17335. doi: 10.1371/journal.pone.0017335

Figure 1. In vitro nuclease assays.

Figure 1

Plasmid DNA (100 ng, pTZ18R) was incubated at 37°C for 1 h with the cellular or supernatant fractions of various S. mutans strains, followed by electrophoresis on an agarose gel. Positive controls: S. aureus (S.a.) and UA159/pVE8009 (+). Negative controls: fresh BHI medium (BHI), cultures from UA159 (159) and UA159/pVE8010 (−). Q, T, S: UA159 derivatives containing LevQ-ΔSPNuc, LevT-ΔSPNuc and LevS-ΔSPNuc fusions, respectively. Open circular (OC), linear (L) and super-coiled (CCC) forms of the plasmid are labeled.