Table 2. Expression of levD promoter:cat fusion as represented by the CAT specific activities in the wild-type strain UA159 and various levT mutants.
Strain | Avg CAT specific activity ± SDa on various growth carbohydrates | |||
Glucose | Fructose | Mannose | Galactose | |
levT+ | 2.8±0.4 | 474.0±44.7 | 870.4±85.2 | 9.6±0.6 |
levT M1stop | 3.9±0.8 | 91.3±10.6 | 83.6±7.6 | 24.8±2.1 |
levT ΔC (sp) | 3.1±0.0 | 0.1±0.0 | 1.2±0.6 | 208.2±13.2 |
levT ΔC (em) | 6.4±0.9 | 0.7±0.4 | 0.6±0.1 | 592.3±36.8 |
levT ΔC (em)/levQ | 0.0±0.0 | 0.0±0.0 | 0.6±0.2 | 0.5±0.3 |
The data are presented as the average results of at least three independent cultures. Cells were cultured in TV broth with 0.5% of the indicated carbohydrates and assays were performed as described in Materials and Methods. Activity is expressed as nmol of chloramphenicol acetylated (mg of protein−1×min−1).