Table 4. Expression of levD promoter:cat fusion in wild type UA159, reconstituted GPS®-LS mutant SLS65 and its derivatives, as measured by CAT assays.
| Strain | Avg CAT sp act SDa on various growth carbohydrates | |||
| Glucose | Fructose | Mannose | Galactose | |
| levS+ | 2.8±0.4 | 474.0±44.7 | 870.4±85.2 | 9.6±0.6 |
| levS LS65 | 1,101±34.3 | 328.3±9.0 | 580.1±32.0 | 1,892.2±11.4 |
| levS LS65/ levQ | 1.3±1.8 | 0.3±0.3 | 2.7±2.1 | 10.2±1.9 |
| levS LS65/ levT M1stop | 204.7±4.1 | 31.0±2.6 | 61.2±1.9 | 106.5±11.3 |
a
The data are presented as the average results of at least three independent cultures. Cells were cultured in TV broth with 0.5% of the indicated carbohydrates and assays were performed as described in Materials and Methods. Activity is expressed as nmol of chloramphenicol acetylated (mg of protein−1×min−1).