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. 2010 Dec 8;84(3):613–618. doi: 10.1095/biolreprod.110.088831

FIG. 3.

FIG. 3.

Generation of viable progeny with two fathers. A) Clone 4E4 XO ES cell-derived female chimera (arrow) and ES cell-derived progeny (agouti pigmented). B) DXMit216 PCR genotype analysis for X chromosome origin in progeny from 4E4 XO ES cell-derived female chimeras. Strain 129 generates a 145-bp fragment; B6 generates a 139-bp fragment. C) Clone 5F7 XO iPS cell-derived female chimera (arrow), B6 father (arrowhead), and iPS cell-derived progeny (agouti pigmented). D) PCR genotyping of iPS-derived progeny for Pou5f1-GFP transgene. The S3 iPS cell line is hemizygous for Pou5f1-GFP; therefore, ∼50% of the progeny inherit the transgene.