Table 5.
Kinetic parameters of human ASL hybrid pairs at 25 °C
| Enzyme | Vmax ± S.Ea (μmol/min/mg) | K0.5 ± S.Ea (μM) | Hill Coefficienta |
|---|---|---|---|
| WT | 9.2 ± 0.1 | 1.9 ± 0.05 | 1.8 ± 0.1 |
| R194C | 12.0 ± 0.9 | 2.2 ± 0.3 | 1.3 ± 0.2 |
| L311V | 8.6 ± 0.7 | 3.4 ± 0.5 | 1.3 ± 0.1 |
| R396H | 1.9 ± 0.2 | 3.8 ± 0.8 | 0.9 ± 0.2 |
| R396C | 2.0 ± 0.2 | 1.1 ± 0.2 | 0.9 ± 0.1 |
| non-His-tag WT/His-tag WT | 9.8 ± 0.3 | 2.2 ± 0.1 | 1.8 ± 0.2 |
| non-His-tag R396C/His-tag WT | |||
| Pool 1 | 2.1 ± 0.3 | 1.0 ± 0.1 | 1.0 ± 0.1 |
| Pool 2 | 4.2 ± 0.1 | 1.1 ± 0.1 | 1.4 ± 0.2 |
| Pool 3 | 9.2 ± 0.7 | 3.2 ± 0.3 | 1.6 ± 0.1 |
| non-His-tag R396H/His-tag WT | |||
| Pool 1 | 1.9 ± 0.1 | 3.2 ± 0.9 | 0.7 ± 0.06 |
| Pool 2 | 5.5 ± 0.2 | 1.9 ± 0.2 | 1.4 ± 0.1 |
| Pool 3 | 9.4 ± 0.6 | 2.7 ± 0.3 | 1.5 ± 0.2 |
| non-His-tag R396C/His-tag R194C | |||
| Pool 1 | 2.2 ± 0.1 | 1.3 ± 0.3 | 0.8 ± 0.1 |
| Pool 2 | 6.0 ± 0.2 | 1.9 ± 0.2 | 1.5 ± 0.2 |
| Pool 3 | 10.4 ± 0.4 | 1.9 ± 0.2 | 1.2 ± 0.1 |
| non-His-tag R396H/His-tag L311V | |||
| Pool 1 | 1.9 ± 0.1 | 3.2 ± 0.9 | 0.7 ± 0.06 |
| Pool 2 | 5.3 ± 0.2 | 1.0 ± 0.1 | 1.2 ± 0.1 |
| Pool 3 | 7.9 ± 0.6 | 2.4 ± 0.4 | 1.1 ± 0.1 |
a
The Vmax, K0.5, and Hill coefficient values were determined by varying the concentration of SAMP and fitting the data to the Hill equation using Sigma Plot. The values are shown along with their standard errors.