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. Author manuscript; available in PMC: 2012 Feb 24.
Published in final edited form as: Vaccine. 2011 Jan 14;29(10):1891–1898. doi: 10.1016/j.vaccine.2010.12.134

Figure 1.

Figure 1

Column chromatography analysis of GalXM conjugates and its components. Absorbance and phenol sulfuric acid reactivity of the reaction mixture of CDAP activated GalXM to carrier proteins and free protein standards. Activated GalXM was conjugated to (A) BSA and (B) PA (open circle and diamond respectively). Same amount of (A) BSA and (B) PA (closed circle and diamond respectively) was used as the standard. Phenol sulfuric acid test of the conjugate mixtures were presented in triangles. Protein and polysaccharide signals were recorded at 280 nm and 485 nm respectively.