Table 1.
Group B N. meningitidis strains used to measure bactericidal activity
| Factor H binding protein (fHbp) |
Neisserial Heparin Binding Antigen (NHba) |
NadA gene6 |
||||||
|---|---|---|---|---|---|---|---|---|
| Strain | Reference | Clonal Complex1 |
ID number2 |
Variant Group3 |
Expression4 | Percent amino acid identity5 |
Expression4 | |
| H44/76-SL | Welsch et al [7, 24] | 32 | 1 | 1 | ++ | 91 | +/− | - |
| M4407 | Giuliani et al [15] | 41/44 | 19 | 2 | + | 100 | ++ | - |
| S3446 | Welsch et al [6] | 41/44 | 19 | 2 | + | 98 | ++ | - |
Clonal complex as defined by multilocus sequence typing [27].
fHbp ID number based on amino acid sequence variant as designated on the website, http://pubmlst.org/neisseria/fHbp/. fHbp ID 1 in strain H44/76 matched the amino acid sequence of the fHbp ID 1 antigen in the vaccine; the amino acid sequence identity between fHbp ID 1 in the vaccine and ID 19 in strains M4407 and S3446 was 72%.
fHbp variant group as classified by Masignani et al [25].
Expression of fHbp and NHba was measured by infrared Western blot [17] (See text).
As compared with the sequence encoded by the gene from strain NZ98/254 [6].
The absence of the NadA gene was confirmed by polymerase chain reaction [14].