Table 2.

Interaction of SHP with other nuclear receptors and transcription factors, or coregulators

Classification	Interacting protein	Methods	Target gene	References
NR	AR	Y2H and GST pull down: rSHP/hAR		Gobinet et al., 2001
	CAR	Y2H and GST pull down: mSHP/mCAR		Seol et al., 1996
		Y2H and GST pull down: mSHP/mCAR, mSHPΔ128–139/mCAR		Park et al., 2004
		GST pull down: mSHP/mCAR	↓CYP2B	Bae et al., 2004
	DAX-1	Co-IP: hSHP/hDAX-1		Iyer et al., 2006
	ERα	Y2H and GST pull down: rSHP/hERα		Johansson et al., 1999
		Y2H, M2H, and GST pull down: SHP/hERα		Johansson et al., 2000
		Y2H and GST pull down: rSHP/ERα		Goodwin et al., 2000
		GST pull down: mSHP/hERα		Klinge et al., 2002
		Co-IP: hSHP/hERα		Iyer et al., 2006
	ERβ	Y2H and GST pull down: rSHP/hERβ		Johansson et al., 1999;
		Y2H, M2H, and GST pull down: SHP/hERβ		Johansson et al., 2000
	ERRα	Y2H, and M2H: hSHP/ERRα, mSHP/ERRα		Sanyal et al., 2002
	ERRβ	Y2H, and M2H: hSHP/ERRβ, mSHP/ERRβ		Sanyal et al., 2002
	ERRγ	Y2H: and M2H: hSHP/ERRγ, mSHP/ERRγ		Sanyal et al., 2002
		Y2H: rSHP/rERRγ		Razzaque et al., 2004
		Y2H and GST pull down: mSHP/ERRγ, mSHPΔ128–139/ERRγ		Park et al., 2004
		GST pull down: hSHP/mERRγ	↓YY1	Zhou et al., 2010
	FXR	M2H and GST pull down: hSHP/mFXR, mSHP/mFXR	↓PCK1	Lu et al., 2000
	GR	Co-IP: rSHP/hGR		Borgius et al., 2002
	HNF4α	M2H and GST pull down: mSHP/rHNF4α		Lee et al., 2000
		M2H and GST pull down: hSHP/rHNF4α, hSHP/hHNF4α		Boulias et al., 2004
		GST pull down: hSHP/hHNF4α	↓AGT ↓FBP1	Shimamoto et al., 2004
		M2H: SHP/HNF4α	↓MTP	Hirokane, et al., 2004
		GST pull down: hSHP/hHNF4α	↓ApoCIII ↓APoB	Zhou et al., 2010
	LRH-1	Y2H: rSHP/mLRH-1 LBD M2H: mSHP/hLRH-1 LBD		Lee et al., 2000
		M2H and GST pull down: mSHP/m LRH-1	↓SHP	Lu et al., 2000
		M2H: SHP/hLRH-1, SHP/mLRH-1 GST pull down: SHP/hLRH-1	↓CYP7A1	Goodwin et al., 2000
		M2H and GST pull down: hSHP/hLRH-1		Lee and Moore, 2002
		Y2H: hSHP/hLRH-1 M2H: mSHP/hLRH-1		Brendel et al., 2002
		M2H: mSHP/mLRH-1		Boulias et al., 2004
		Y2H and GST pull down: mSHP/ LRH1, mSHPΔ128–139/LRH1		Park et al., 2004
		SHP/LRH-1	↓MTP	Huang et al., 2007
		SHP/LRH-1	↓cyclinD1	Zhang et al., 2008
		GST pull down: hSHP/mLRH-1	↓SHP	Zhou et al., 2010
	LXRα	GST pull down: mSHP/hLXRα		Brendel et al., 2002
	LXRβ	GST pull down: mSHP/mLXRβ		Brendel et al., 2002
	Nuf77	M2H and Co-IP: SHP/Nuf77	↓CYP17	Yeo et al., 2005
	PPARα	Y2H: rSHP/r PPARα LBD GST pull down: rSHP/rPPARα		Kassam et al., 2001
	PPARγ	M2H and GST pull down: hSHP/hPPARα		Nishizawa et al., 2002
	PXR	GST pull down: mSHP/mPXR, mSHP/hPXR	↓CYP3A	Ourlin et al., 2003
	RARα	Y2H: mSHP/RARαLBD M2H: hSHP/hRARαLBD		Seol et al., 1996
		Y2H: mSHPΔ148/mRARαLBD		Seol et al., 1997
	RXRα	Y2H, M2H, and GST pull down: mSHP/hRXRα		Seol et al., 1997
		M2H and GST pull down: mSHP/mRXRα		Goodwin et al., 2000
	SF-1	M2H: hSHP/mSF-1LBD GST pull down: mSHP/mSF-1		Lu et al., 2000
	SHP	Co-IP: SHP/SHP		Iyer et al., 2007
	TRβ	Y2H: mSHP/TRβ LBD Y2H: hSHP/hTRβ LBD		Seol et al., 1996; Seol et al., 1997
TF (Non-NR)	ARNT	GST pull down: SHP/ARNT	↓CYP1A1 ↓UGT1A6	Klinge et al., 2001
	BETA2/NeuroD	Y2H and GST pull down: SHP/BETA2	↓βGK ↓p21	Kim et al., 2004
	C-jun	Co-IP: rSHP/rC-jun		Fiorucci et al., 2004
	Jun D	Co-IP: rSHP/rJun D		Fiorucci et al., 2004
	C/EBPα	M2H and GST pull down: SHP/N-terminal domain of C/EBPα Co-IP: SHP/C/EBPα	↓PEPCK	Park et al., 2007
	Foxo1	GST pull down: SHP N1 or NT/Foxo1 CT Co-IP: SHP/Foxo1	↓G6Pase	Yamagata et al., 2004
	HNF3	GST pull down and Co-IP: SHP/HNF3α, SHP/HNF3β, SHP/HNF3γ	↓G6Pase ↓CYP7A1	Kim et al., 2004
	NF-κB	Y2H, GST pull down and Co-IP: SHP/p65		Kim et al., 2001
	Smad3	Y2H, GST pull down and Co-Ip: SHP/Smad3	↓TGFβ ↓p21 ↓smad7 ↓PAI-1	Suh et al., 2006
	Bcl2	Co-IP: mSHP/hBcl2		Zhang et al., 2010
	Gli	GST pull down and Co-IP: SHP/Gli	↓PTCH1 ↓BCl2 ↓BClxL	Kim et al., 2010
	P53	GST pull down and Co-IP: SHP/P53	↓miR34a	Lee et al., 2010
Co-regulator
Co-repressor	BAF155	GST pull down: mSHP/BAF155	↓CYP7A1	Kemper et al., 2004
	BAF57	GST pull down: mSHP/BAF57	↓CYP7A1	Kemper et al., 2004
	Brm	GST pull down and Co-IP: mSHP/Brm	↓CYP7A1	Kemper et al., 2004
		Co-IP: mSHP/Brm	↓CYP7A1 ↓SHP	Miao et al., 2009
	EID1	Y2H, M2H, GST pull down and Co-IP: rSHP/mEID1		Bavner et al., 2002
		Y2H and GST pull down: mSHP/EID1		Park et al., 2004
		GST pull down: hSHP/mEID1		Zhou et al., 2010
	G9a	GST pull down and Co-IP: SHP/G9a		Boulias et al., 2004
		GST pull down and Co-IP: SHP/G9a	↓CYP7A1 ↓CYP8B1	Fang et al., 2007
	HDAC1	GST pull down: SHP/HDAc1	↓CYP2B	Bae et al., 2004
		GST pull down and Co-IP: SHP/HDAC1		Boulias et al., 2004
		GST pull down and Co-IP: SHP/HDAC1		Gobinet et al., 2005
	HDAC3	GST pull down: SHP/HDAC3		Gobinet et al., 2005
	HDAC5	GST pull down: SHP/HDAC5		Gobinet et al., 2005
	HDAC6	GST pull down: SHP/HDAC6		Gobinet et al., 2005
	mSin3A	GST pull down and Co-IP: mSHP/mSin3A	↓CYP7A1	Kemper et al., 2004
		GST pull down: SHP/mSin3A	↓CYP2B	Bae et al., 2004
	SMRT	GST pull down: SHP/SMRT		Bae et al., 2004
		GST pull down: SHP/SMRT	↓CYP2B	Bae et al., 2004
	SIRT1	M2H, GST pull down and Co-IP: SHP/SIRT1	↓CYP7A1 ↓SHP	Chanda et al., 2010
Co-activator	CBP/p300	Co-IP: SHP/CBP		Yeo et al., 2005
		M2H and GST pull down: SHP/p300	↓TGFβ ↓p21 ↓smad7 ↓PAI-1	Suh et al., 2006
	SRC-1	GST pull down: SHP/SRC-D (759–1141), SHP/SRC-E (1101–1441)		Kim et al., 2001

Abbreviations: NR, Nuclear receptor; TF, Transcription factor; Co-IP, coimmunoprecipitation assay; M2H, mammalian two hybrid system; Y2H, yeast two-hybrid system; h, human; m, mouse; r, rat.