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DEER evidence for a SOD1-induced conformational change in ExoU. (A and C) Dipolar evolution curves (black) and fits (red) obtained using model-free Tikhonov regularization for the S137R1-S643R1 rExoU double cysteine variant in the (A) absence and (C) presence of SOD1. (B and D) Corresponding distance distributions in the (B) absence and (D) presence of SOD1. The regularization parameter (α) was 10 for both fits. L-curves are given in Fig. S8.
