Table 2.
Fluorescence lifetimes of AlexaFluor 430 and the AlexaFluor 430-labeled melittin mutant at an interface in situ
| Fluorescence lifetimes from TREWIFS measurements | |||||
|---|---|---|---|---|---|
| System | amp(τ1) | τ1(ns) | amp(τ2) | τ2(ns) | χ2 |
| AlexaFluor 430-SiO2 | – | – | 100 | 3.1 | 1.03 |
| Peptide-SiO2 | 57 | 0.9 | 43 | 3.3 | 1.17 |
| Peptide-bilayer | 40 | 1.2 | 60 | 3.7 | 1.16 |
The system indicates the molecule and the surface to which it adsorbs or with which it interacts. The fluorescence decay kinetics of AlexaFluor 430 is single-exponential, whereas that of the peptide is biexponential; therefore, the percentage contribution of the two lifetime components is given. χ2 is the goodness of fit to the decay curves.