Figure 7.

Anionic supramolecular assemblies have a limited capacity to inhibit SEVI-mediated enhancement of HIV-1 infection. (A) SEVI fibrils (5 μM) were allowed to form a complex with HIV-1 virions for 10 min at room temperature. The anionic peptide supramolecular assemblies were then added to the SEVI:virus solution and incubated for 10 min. This solution was then incubated on CEMx M7 cells. After 48 h, the cells were harvested and luciferase expression measured as a readout of HIV-1 infection. (B) HIV-1 infections were performed on CEMx M7 cells in the presence of increasing concentrations of E₄(ChaKChaE)₂ alone, in the absence of SEVI. Results in both panels represent mean values from three experimental replicates; error bars denote the standard deviation of these values. The results shown are representative of three independent experiments performed with a range of anionic peptide concentrations all yielding similar results.