Table 2.
Amplified fragment length polymorphism (AFLP) adapters and primers used in the ligation and amplification steps, and their sequences
| Adapters | EcoRI adapters | 5′-CTCGTAGACTGCGTACC-3′ |
| 3′-CTGACGCATGGTTAA-5′ | ||
| MseI adapters | 5′-GACGATGAGTCCTGAG-3′ | |
| 3′-TACTCAGGACTCAT-5′ | ||
| PstI adapters | 5′-CTCGTAGACTGCGTACATGCA-3′ | |
| 3′-TGTACGCAGTCTAC-5′ | ||
| Universal primers | E00 | 5′-GACTGCGTACCAATTC-3′ |
| M00 | 5′-GATGAGTCCTGAGTAA-3′ | |
| P00 | 5′-GACTGCGTACATGCAG-3′ | |
| Primer pairs in the pre-amplification | ||
| E01 (E00+A) - 02 (M00+C) | ||
| P01 (P00+A) - M01 (M00+A) | ||
| P01 (P00+A) - M02 (M00+C) | ||
| Primer pairs in the selective amplification | ||
| E35 (E00+ACA) | M52A (M00+CCCA) | |
| M52C (M00+CCCC) | ||
| M52T (M00+CCCT) | ||
| E36 (E00+ACC) | M52C (M00+CCCC) | |
| E37 (E00+ACG) | M52A (M00+CCCA) | |
| M52C (M00+CCCC) | ||
| M52G (M00+CCCG) | ||
| E38 (E00+ACT) | M52G (M00+CCCG) | |
| M52T (M00+CCCT) | ||
| P31 (P00+AAA) | M33 (M00+AAG) | |
| M35 (M00+ACA) | ||
| P35 (P00+ACA) | M32 (M00+AAC) | |
| M33 (M00+AAG) | ||
| M34 (M00+AAT) | ||
| M35 (M00+ACA) | ||
| M36 (M00+ACC) | ||
| M47 (M00+CAA) | ||
| M50 (M00+CAT) | ||
| P38 (P00+ACT) | M47 (M00+CAA) | |
| M48 (M00+CAC) | ||
| P43 (P00+ATA) | M36 (M00+ACC) | |
| M51 (M00+CCA) | ||