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Gradient mobile phase used during the analysis by HPLC-PDA for fluoroquinolones in group A and group B.
| Time (min) | Methanol (%) | Acetonitrile (%) | Buffer (20 mM) (%) | Flow rate (mL/min) |
|---|---|---|---|---|
| Group A | ||||
| 0.01 | 0 | 10 | 90 | 1.0 |
| 4.00 | 5 | 15 | 85 | 1.0 |
| 10.0 | 0 | 40 | 60 | 1.0 |
| 12.0 | 0 | 10 | 90 | 1.0 |
| Group B | ||||
| 0.01 | 10 | 10 | 80 | 1.0 |
| 6.00 | 10 | 10 | 80 | 1.0 |
| 8.00 | 0 | 50 | 50 | 1.0 |
| 10.0 | 10 | 10 | 80 | 1.0 |
