Fig. 2.

Toll-like receptor (TLR)-4, myeloid differentiation factor 88 (MyD88) and myeloid differentiation protein 2 (MD-2) secretion in THP-1 (human acute monocytic leukaemia) cells stimulated with anti-β₂-glycoprotein I/β₂-glycoprotein I (anti-β₂GPI/β₂GPI) complex. The THP-1 cells (2 × 10⁶) were treated with anti-β₂GPI (10 µg/ml)/β₂GPI (100 µg/ml) complex, isotype control rabbit immunoglobulin G (R-IgG) (10 µg/ml)/β₂GPI (100 µg/ml) and lipopolysaccharide (LPS) (500 ng/ml) for 2 h (a) or 6 h (b). TLR-4, MyD88 and MD-2 mRNA levels (a) were detected by real-time quantitative reverse transcription–polymerase chain reaction (qRT–PCR), and their protein levels (b) were analysed by Western blotting. Data shown are from three independent experiments. *P < 0·05 versus control of untreated cells.