Fig. 5.

Paclitaxel inhibited anti-β₂-glycoprotein I/β₂-glycoprotein I (anti-β₂GPI/β₂GPI)-stimulated tissue factor (TF) expression on THP-1 (human acute monocytic leukaemia) cells. The THP-1 cells were pretreated with paclitaxel (1 µM) for 1 h, then stimulated by anti-β₂GPI (10 µg/ml)/β₂GPI (100 µg/ml) complex, isotype control rabbit immunoglobulin G (R-IgG) (10 µg/ml)/β₂GPI (100 µg/ml) and lipopolysaccharide (LPS) (500 ng/ml) for 2 h (a) or 6 h (b). The TF mRNA (a) and TF activity (b) were measured by real-time quantitative reverse transcription–polymerase chain reaction (qRT–PCR) and TF activity kits, respectively. Data shown are from three separate experiments. **P < 0·01 versus no paclitaxel-treated cells.