Fig. 7.

Toll-like receptor (TLR)-4, myeloid differentiation factor 88 (MyD88) and myeloid differentiation protein 2 (MD-2) expression in lentiviral-infected THP-1 (human acute monocytic leukaemia) cells. The THP-1 cells infected with LV-GFP or LV-RNAi-annexin A2 (ANX2) were incubated with anti-β₂-glycoprotein I (β₂GPI) (10 µg/ml)/β₂GPI (100 µg/ml) for 2 h or 6 h. The mRNA levels and the protein expression of TLR-4 (a1, a2), MyD88 (b1, b2) and MD-2 (c1, c2) were detected by real-time quantitative reverse transcription–polymerase chain reaction (qRT–PCR) and Western blotting. Data shown are from three separate experiments. *P < 0·05 versus no lentivirus cells.