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Expression of two prostaglandin D2 (PGD2) receptors in the middle ear. RNA was extracted from middle ear mucosa in wild-type mice 3 h and 24 h after phosphate-buffered saline (PBS) or lipopolysaccharide (LPS) injection. Messenger RNA for D prostanoid receptor (DP) and chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) were detected by reverse transcription–polymerase chain receptor. M, molecular marker.
