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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Prostate. 2010 Aug 31;71(4):333–343. doi: 10.1002/pros.21247

Fig. 2.

Fig. 2

DSF treatment leads to de-methylation of methylated promoter regions in prostate cancer cells and results in re-expression of RARB and APC. A: C4-2B and CWR22Rv1 cells were cultured for 2 or 4 weeks in the presence of 0.5 μMDSF (C4-2B for RARB and CWR22Rv1 cells for APC). Total DNA was extracted and promoter methylation status was evaluated with methylation-specific PCR (MSP) using primers specific to the methylated (M) and unmethylated (U) sequence after bisulfite conversion. An increase in the signal with unmethylated-specific primers indicated de-methylation of the locus. B: RNA was extracted from CWR22Rv1 cells treated with 0.5 μMDSF or solvent control for 2 or 4 weeks. APC and RARB transcript levels were determined by quantitative real-time PCR and are shown as relative expression levels normalized to TBP expression.