Fig. 4.

DSF does not affect per-cell PSA secretion. A: PSA producing C4-2B cells were exposed to indicated concentrations of DSF. The conditioned media as well as the total cell lysates were collected after 48 hr and PSA concentrations were determined by ELISA. B: C4-2B cells were exposed to 0.5 μM disulfiram. After 2 weeks PSA concentrations in conditioned medium as well as the cell number of surviving cells were determined. Graph shows PSA levels normalized to cell number in solvent control and DSF-treated cells. C: Serum samples from C4-2B xenograft mice (see Fig. 3D) were collected and PSA concentration was determined by ELISA. Graph shows relative PSA levels normalized to tumor weights.