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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biomaterials. 2011 Jan 22;32(11):3053–3061. doi: 10.1016/j.biomaterials.2010.12.036

Fig. 6.

Fig. 6

Endocrine-like activity of modified MSCs. (A) Schematic illustration of programming cell fate of distant cells (without particles in well ‘ii’) by transferring conditioned media from well ‘i’, containing DEX-PLGA modified MSCs, differentiated MSCs, or DEX-PLGA modified fibroblasts to well ‘ii’. (B) Osteogenic differentiation of DEX-PLGA modified MSCs and distant cells quantified through ALP staining. (C) Osteogenic differentiation of MSCs treated with conditioned media from differentiated MSCs without DEX-PLGA particles. (D) Osteogenic differentiation of MSCs treated with conditioned media from DEX-PLGA modified fibroblasts. (E) Schematic illustration of DEX-PLGA modified MSCs controlling the fate of MSCs (without particles) separated by a transwell membrane 2 mm above the surface. (F) Osteogenic differentiation of unmodified MSCs atop transwell membrane quantified through ALP staining. D=Dexamethasone, A=Ascorbic Acid, G=β-Glycerolphosphate, CCM=MSC expansion media