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. 2011 Feb;49(2):732–734. doi: 10.1128/JCM.01970-10

Fig. 1.

Fig. 1.

(A) Duplex PCR for CC398 sau1-hsdS1 and mecA detection. Lane M, 100-bp DNA ladder; lane 1, negative control (water); lane 2, non-CC398 methicillin-susceptible S. aureus (strain ATCC 6538); lane 3, non-CC398 methicillin-resistant S. aureus (strain ATCC 33591); lane 4, CC398 methicillin-sensitive S. aureus (SSI 52615); lane 5, CC398 methicillin-resistant S. aureus (KVL 288). (B) Real-time PCR melting curve analysis showing the melting temperature (Tm) of the amplicons. (−R′(T)), negative derivative of fluorescence with respect to temperature.