Table 1.
S. aureus strain collection used for validation of the CC398-specific PCR
Origin (no. of isolates) | Clonal complexb | spa type(s) (no. of isolates where ≥10) | No. of isolates of indicated clonal complex type | No. of sau1-hsdS1 PCR-positive results |
---|---|---|---|---|
Cattle (8) | CC80 | t527 | 1 | 0 |
CC9 | t2839 | 1 | 0 | |
NDa | t524, 2873, t3046 | 6 | 0 | |
Dog (9) | CC15 | t084, t774 | 2 | 0 |
CC20 | t091 | 1 | 0 | |
CC25 | t227 | 1 | 0 | |
CC30 | t3055 | 1 | 0 | |
CC5 | t548 | 1 | 0 | |
CC8 | t030 | 1 | 0 | |
ND | t1335, t1651 | 2 | 0 | |
Goat (1) | ND | t1166 | 1 | 0 |
Horse (13) | CC15 | t084 | 1 | 0 |
ND | t1166, t1294, t2112, t2484, t3043, t3044 | 12 | 0 | |
Human (115) | CC22 | t005, t022, t032, t223, t541 | 10 | 0 |
CC30 | t012, t016, t019, t318 | 10 | 0 | |
CC398 | t011, t034 (35), t108, t567, t571 | 40 | 40 | |
CC45 | t015, t065, t230 | 7 | 0 | |
CC5 | t001, t002, t003, t041, t045 | 20 | 0 | |
CC8 | t008, t024, t037, t064 | 21 | 0 | |
CC80 | t044, t376 | 5 | 0 | |
ST152/377 | t355 | 2 | 0 | |
Poultry (114) | CC5 | t002 (64), t306, t2049 | 66 | 0 |
CC8 | t304 | 1 | 0 | |
CC9 | t1430 | 19 | 0 | |
CC80 | t203 | 1 | 0 | |
CC398 | t011 (11), t034, t108, t567, t1456, t4652 | 25 | 25 | |
ND | t324, t2038 | 2 | 0 | |
Sheep (5) | ND | t2678, t3042, t3045, t3047 | 5 | 0 |
Swine (1,042) | CC1 | t127 | 2 | 0 |
CC5 | t151, t2164 | 2 | 0 | |
CC30 | t1333, t2840 | 8 | 0 | |
CC398 | t011 (404), t034 (13), t108 (575), t571, t899, t1255, t1344, t1793, t2330, t2876 | 1,007 | 1,007 | |
CC9 | t337 (21), t899c, t2839 | 23 | 0 |
ND, not determined (but non-CC398).
Clonal complex associations were determined using http://spaserver.ridom.de and by comparing the results of MLST mapping of previous isolates with similar or related spa types with subsequent clustering by the use of eBURST v3 (http://eburst.mlst.net).
MLST typed.