Table 1.
Primer sequences for the H-antigen PCR assay
| Primer name | Concn (μmol) | Sequence |
|---|---|---|
| 1EN_F1 | 1 | CGCTGAACGTGCAGAAAGAGTATGATGT |
| 1EN_F2 | 1 | AACGTGCAGAAAAAATATGATGT |
| ri_F | 1 | GTGCAACAAAAATATAAGGTCAGC |
| bd_F | 1 | GCGAACGACGGTGAAACTAT |
| G_F | 1 | TCCAGCTTCAAGAATGTTACGGG |
| z51_F | 1 | ATCTAATTTCAAAAACGTTACTGG |
| z29_F | 1 | AAAAAGCCTTGGAATGGATGG |
| Z4_F | 1 | AGCTGGGCTTAGATAAATTAGATGT |
| L_F | 1 | GCAGAAAAAATATGATGTGAAGAGC |
| 1ENL_R | 5 | AACTGCCTTCAATTGTCTTACC |
| ri_R | 5 | CACCCGCTGCTGTCAATG |
| b_R | 5 | CGGTCACCTCAACGAAGTAG |
| G_R | 5 | TATAAACATTTTTGCTTGATTGTAAGG |
| mt_R | 5 | TAGCAGTATATTCAGCTCCCATT |
| c_R | 5 | ATTTATTCGTCAGCAGTTTTTTC |
| y_R | 5 | AACCGCCTTCAATTGTTTTACC |
| eh_R | 5 | CGTCAATAGATTTTCCATTTTTATC |
| z29_R | 5 | CCGCGTTAACAAATGACAGC |
| Z4_R | 5 | GGCAGATTCAAAACGGTTC |
| d_R | 5 | GCATAGCCACCATCAATAACC |