Table 4.

Evaluation of the H antigen assay on 500 isolates

Description	Explanation	No. of isolates	% of total
Identified as expected
Matched traditional serotyping	Molecular results matched traditional results completely	402	80.4
No probe for one or more antigens	Possessed an H antigen not detected by the probes currently in the assay, resulting in partial serotype determination	46	9.2
Monophasic isolates	Monophasic by traditional methods; two H antigens detected by molecular method; includes 4 serotype Paratyphi A strains	9	1.8
Nonmotile isolates	Nonmotile variant; H antigen detected in molecular assay matched a common serotype.	4	0.8
Subtotal		461	92.2
Not identified as expected
H:m,t and -g,s,t	Did not react with t-1 probe; allelic diversity suspected	19	3.8
H:1,5	Did not react with 5-1 probe; allelic diversity suspected	6	1.2
H:z	All are subspecies IIIb serotypes	6	1.2
H:m,t	Salmonella serotype Pensacola; did not react with m/m,t probe	2	0.4
H:l,v	Salmonella serotype I 9,12:l,v:−; did not react with v probe	2	0.4
H:d	Salmonella serotype Putten; shown to have a divergent H:d allele	1	0.2
H:1,5,7	Did not react with 5-1 or 7 probe; subspecies II strain	1	0.2
H:e,n,x,z15	Did not react with x probe; subspecies II strain	1	0.2
H:z35	Subspecies IIIb strain	1	0.2
Subtotal		39	7.8
Total		500