Table 1.
Primers and probes used in the study
| Primer or probe | Sequence (5′–3′)a | Positions (bp)b |
|---|---|---|
| PPNG-F1 | AATTCATTTAAAAAATCAGATTTTGAGCCTA | 671–701 |
| PPNG-R1 | ACGAAAGTTACCAATGAAGATTTGC | 774–750 |
| PPNG-TM1 | FAM-ATCTATTGCTATCGTTACCCGCTAGAAATACCCAG-BHQ | 708–742 |
| PPNG-F2 | AGCTGTTCGTTTTTTACTACCAATCA | 937–962 |
| PPNG-R2 | TGATTTAGTCGTTGAGGTTGAACAA | 1024–1000 |
| PPNG-TM2 | FAM-AATTTAAAGAGTGAATAGTACGCCCACGCTTGA-BHQ | 966–998 |
a
FAM, 6-carboxyfluorescein; BHQ, black hole quencher.
b
Asian plasmid (GenBank accession number U20374).