Table 1.
Primers used in this study
| Primer | Sequence (positions) | Target and/or purpose (amplified fragment size) | Reference |
|---|---|---|---|
| 8F16S | 5′-AGAGTTTGATCCTGGCTCAG-3′ (8–27)a | 16S rRNA gene, PCR (ca. 1,500 bp), sequencing | 17 |
| 1047R16S | 5′-TGCACACAGGCCACAAGGGA-3′ (1047–1028)a | ||
| 830F16S | 5′-GTGTGGGTTTCCTTCCTTGG-3′ (830–849)a | ||
| 1542R16S | 5′-AAGGAGGTGATCCAGCCGCA-3′ (1542–1523)a | ||
| TB11 | 5′-ACCAACGATGGTGTGTCCAT-3′ | hsp65, PCR (441 bp), sequencing | 19 |
| TB12 | 5′-CTTGTCGAACCGCATACCCT-3′ | ||
| MabrpoF | 5′-GAGGGTCAGACCACGATGAC-3′ (2112–2131)b | rpoB, PCR (449 bp), sequencing | This study |
| MabrpoR | 5′-AGCCGATCAGACCGATGTT-3′ (2559–2541)b | ||
| ITSF | 5′-TTGTACACACCGCCCGTC-3′ | 16S–23S ITS region, PCR (ca. 340 bp), sequencing | 14 |
| ITSR | 5′-TCTCGATGCCAAGGCATCCACC-3′ | ||
| OPA2 | 5′-TGCCGAGCTG-3′ | RAPD-PCR | 25 |
| OPA18 | 5′-AGGTGACCGT-3′ | ||
| INS-2 | 5′-GCGTAGTGCGTCGGTGACAAA-3′ |
a
Nucleotide positions were assigned using the Escherichia coli 16S rRNA gene sequence as a reference.
b
Primer design and nucleotide positions were based on the M. tuberculosis rpoB gene sequence (GenBank/EMBL/DDBJ accession no. L27989).